Antigen

Antigen Class

TFs and others

Antigen

Xbp1

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BJ/L6 background

genotype

Xbp1 flx/flx (WT, wild-type)

gender

male

age

12-16 weeks

tissue

Liver

time point

CT04

antibody

anti-XBP1s (Biolegend Poly6195)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The comprehensive CT0 and CT8 DNA libraries were prepared at the MD Anderson Cancer Center, which generated ~40 million 75 bp paired-end reads per samples. The CT0 to CT36 time point DNA libraries were prepared at the University of Houston Sequencing Core and generated ~30 million 75 bp single-end reads per sample. DNA libraries generated from 1ng input DNA were prepared with KAPA Hyper Prep Kit (KAPABIOSYSTEMS) for the comprehensive CT0 and CT8 DNA libraries and with Ovation® Ultralow V2 DNA-Seq library preparation kit (NuGen) for the CT0 to CT36 time point DNA libraries. The size selection was performed using SPRIselect beads (Beckman Coulter), followed by quality control analyzed using the High Sensitivity DNA chip on Bioanalyzer 2100 (Agilent). The DNA libraries were pooled and sequenced using NextSeq 500 (Illumina).

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

45459022

Reads aligned (%)

96.5

Duplicates removed (%)

4.0

Number of peaks

242 (qval < 1E-05)

mm9

Number of total reads

45459022

Reads aligned (%)

96.4

Duplicates removed (%)

4.1

Number of peaks

205 (qval < 1E-05)